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KMID : 0361019980410111446
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Korean Journal of Otolaryngology - Head and Neck Surgery
1998 Volume.41 No. 11 p.1446 ~ p.1453
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Amplification of int-2 in Head and Neck Squamous Cell Carcinomas and Adjacent Mucosa.
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Jang Il-Hwan
Jung Kwang-Yoon
Oh Seung-Chul
Seok Youn-Sik
Choi Geon
Woo Jeong-Soo
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Abstract
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Background and Objectibes: It is important to identify potential biomarkers of tumorigenesis that can be utilized on histologically normal epithelia to determine the level of risk of tumor development. With the goal of possibly identifying a biomarker for the process of development of head and neck cancer, the amplification of int-2 was observed in patients with head and neck squamous cell carcinoma.
Material and Methods: Fluorescence in situ hybridization using cosmid int-2 probe was performed on paraffin-embedded specimens from tumor and tumor-adjacent and tumor-distant epithelia of 20 patients. Buccal mucosa of cancer-free subjects who smoked and did not smoke cigarettes were used as control. Dot blot hybridization using digoxigenin-labeled int-2 probe was also performed on the frozen tissue from tumor and tumor-adjacent epithelia of 14 patients.
Results: In in situ hybridization, buccal epithelia of cancer-free subjects who smoked and did not smoke cigarettes, and tumor-distant epithelia of the patients with head and neck squamous cell carcinoma showed no int-2 amplification. However, eleven of tumor tissue (55%) and five of tumor-adjacent epithelia (25%) in 20 cases showed int-2 amplification. In dot blot hybridization, five tumor tissue (35.7%) and 2 tumor-adjacent epithelia (14.3%) in 14 cases, of which tumor tissue were all found to have int-2 amplification, showed int-2 amplification.
Conclusion: The amplification of int-2 in the tumor tissue and the tumor-adjacent epithelia of the same cases supports the concept of field cancerization or clonal extension. Such genotype parameters may provide a genetic basis for the development of early recurrence or second primary tumors after therapeutic treatment of head and neck squamous cell carcinomas.
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KEYWORD
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int-2 amplification, Head and neck cancer, in situ hybridization, Dot blot hybirdization
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